Hydroquinone was purchased from Eastman Kodak Company (Rochester, NY, USA, #105 0350). Click on underlined terms for definitions or go to the Pesticide Tutorial overview page. The VWR Traceable Logger-Trac Temperature Datalogger is perfect for monitoring material during storage, handling, and transportation. Primary fixative A (4% paraformaldehyde, 2% glutaraldehyde, ∼100 mM sodium cacodylate, pH 7.4): Prepared freshly by diluting 200 mM sodium cacodylate, pH 7.4, 16% (wt/vol) paraformaldehyde (Electron Microscopy Sciences Cat. The acute toxicity reported on this page is ScienceDirect ® is a registered trademark of Elsevier B.V. ScienceDirect ® is a registered trademark of Elsevier B.V. URL: https://www.sciencedirect.com/science/article/pii/S0091679X08910049, URL: https://www.sciencedirect.com/science/article/pii/B9780123918574000100, URL: https://www.sciencedirect.com/science/article/pii/B978012385116100011X, URL: https://www.sciencedirect.com/science/article/pii/S0091679X10960056, URL: https://www.sciencedirect.com/science/article/pii/B9780128010754000148, URL: https://www.sciencedirect.com/science/article/pii/B9780121852795500091, URL: https://www.sciencedirect.com/science/article/pii/S0076687916303536, URL: https://www.sciencedirect.com/science/article/pii/B978012391860400001X, URL: https://www.sciencedirect.com/science/article/pii/B9780122437403500090, URL: https://www.sciencedirect.com/science/article/pii/B9780123877840000110, Jovenal T. SanAgustin, ... Gregory J. Pazour, in, Imaging and Spectroscopic Analysis of Living Cells, Alexander A. Mironov, Galina V. Beznoussenko, in, The Unfolded Protein Response and Cellular Stress, Part A, Correlative Light and Electron Microscopy II, Molecular Characterization of Autophagic Responses, Part B, Yla-Anttila, Vihinen, Jokitalo, & Eskelinen, 2009, Higaki et al., 2015; Seyedhosseini, Ellisman, & Tasdizen, 2013, Cogliati, Enriquez, & Scorrano, 2016; Rimessi et al., 2015; Scorrano et al., 2002, Anne L. van de Ven, ... Rita E. Serda, in, Enzymology Primer for Recombinant DNA Technology, Mutation Research/Genetic Toxicology and Environmental Mutagenesis. Dehydrate the pellets in graded steps of ethanol (50%, 70%, 90%, and 100%) two times with 100% acetone (each of 15–30 min at 4°C). The full text of this article hosted at iucr.org is unavailable due to technical difficulties. 124-65-2 (CAS number) Cacodylate buffer: 0.1 M sodium cacodylate, pH 7.4. If you do not receive an email within 10 minutes, your email address may not be registered, Precipitates usually do not occur. Stir continuously until paraformaldehyde powder goes into solution. For DNA with 3′-protruding ends, a dNTP/DNA ratio of ∼20 is optimal. Make from 25% stock (Electron Microscopy Sciences). , This carefully selected portfolio is specifically designed to help you prevent potential contamination and maintain aseptic conditions in cleanrooms and controlled environments... A pre-moistened cleansing wipe for the face or hands, ideal for gown rooms. O-F solution: 1% osmium tetroxide, 1.5% potassium ferrocyanide trihydrate in 0.1 M sodium cacodylate, pH 7.4. Day of induction On the day of treatment, the culture should not exceed 106 cells/ml. Discard the supernatant into OsO4 neutralizer and use the neutralizer also to wash the plastic Pasteur pipettes before discarding them to the toxic waste. Hyone-Myong Eun, in Enzymology Primer for Recombinant DNA Technology, 1996. II. Unlimited viewing of the article PDF and any associated supplements and figures. High-quality chemicals and services, customized to your product or manufacturing needs... VWR enables the advancement of science by providing high-quality chemicals and services, customized to your product or manufacturing needs. Class IV mitochondria: High morphological derangements, consisting in definitively swollen and ruptured mitochondria with undistinguishable cristae structure.
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